| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories
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| Autores: |
Gatesoupe, Francois-joel
Huelvan, Christine
Le Bayon, Nicolas
Le Delliou, Herve
Madec, Lauriane
Mouchel, Olivier
Quazuguel, Patrick
Mazurais, David
Zambonino-infante, Jose-luis
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| Data: |
2016-11
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| Ano: |
2016
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| Palavras-chave: |
Host-microbe interaction
16S rRNA
Pyrosequencing
Autochthonous bacteria
Alternative feed ingredients
Physiological status
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| Resumo: |
Background The better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable aquaculture development. The present experiment aimed at correlating active microbiota associated to intestinal mucosa with Specific Growth Rate (SGR) and Hypoxia Resistance Time (HRT) in European sea bass individuals submitted to different nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the dietary challenge in a common garden approach at the juvenile stage. Results A diet deficient in essential fatty acids (LH) lowered both SGR and HRT in sea bass, especially when the deficiency was already applied at first feeding. A protein-deficient diet with high starch supply (HG) reduced SGR to a lesser extent than LH, but it did not affect HRT. In overall average, 94 % of pyrosequencing reads corresponded to Proteobacteria, and the differences in Operational Taxonomy Units (OTUs) composition were mildly significant between experimental groups, mainly due to high individual variability. The highest and the lowest Bray-Curtis indices of intra-group similarity were observed in the two groups fed standard starter diet, and then mixed before the final dietary challenge with fish already exposed to the nutritional deficiency at first feeding (0.60 and 0.42 with diets HG and LH, respectively). Most noticeably, the median percentage of Escherichia-Shigella OTU_1 was less in the group LH with standard starter diet. Disregarding the nutritional history of each individual, strong correlation appeared between (1) OTU richness and SGR, and (2) dominance index and HRT. The two physiological traits correlated also with the relative abundance of distinct OTUs (positive correlations: Pseudomonas sp. OTU_3 and Herbaspirillum sp. OTU_10 with SGR, Paracoccus sp. OTU_4 and Vibrio sp. OTU_7 with HRT; negative correlation: Rhizobium sp. OTU_9 with HRT). Conclusions In sea bass, gut microbiota characteristics and physiological traits of individuals are linked together, interfering with nutritional history, and resulting in high variability among individual microbiota. Many samples and tank replicates seem necessary to further investigate the effect of experimental treatments on gut microbiota composition, and to test the hypothesis whether microbiotypes may be delineated in fish.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00356/46719/46590.pdf
DOI:10.1186/s12866-016-0885-2
https://archimer.ifremer.fr/doc/00356/46719/
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| Editor: |
Biomed Central Ltd
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/288925/EU//ARRAINA
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| Formato: |
application/pdf
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| Fonte: |
Bmc Microbiology (1471-2180) (Biomed Central Ltd), 2016-11 , Vol. 16 , N. 266 , P. 1-13
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| Direitos: |
The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver...
info:eu-repo/semantics/openAccess
restricted use
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